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Product category: Optical Filters, Lenses, Gratings etc
News Release from: Semrock | Subject: BrightLine filters
Edited by the Electronicstalk Editorial Team on 16 May 2005

Fluorescence filters work
with UV sources

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Semrock has developed a new suite of fluorescence filter sets specifically for use with UV-excitation sources

These exceptional new filter sets, including a Fura-2 set for fast Ca2+ imaging, a low-noise high-sensitivity DAPI set, and a high-performance multiband 'Pinkel' set, are all in stock for quick shipment. Semrock's new four-filter Fura-2 set is specially designed to achieve high-performance and fast, but highly accurate, ratiometric imaging of Ca2+.

The carefully optimised excitation filters provide superb balance of free and saturated Ca2+ signals, while minimising crosstalk and background noise.

The exceptionally high throughput of this filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output.

Independent laboratories have measured this set to have from two to four times the free and saturated signals as filters from the leading competitor.

A new high-sensitivity filter set for the blue fluorophores DAPI and Hoechst is optimised for achieving very low noise (background) yet exceptionally high brightness, resulting in the highest contrast (signal-to-noise ratio) set on the market.

It also provides low phototoxicity and low photobleaching of other fluorophores in multicolour systems.

The overall throughput is as much as 4 times that of the leading competitor's DAPI set, while providing as much as 50% higher contrast.

This new set is the unparalleled choice for most applications, especially those involving electronic imaging, and is an excellent complement to Semrock's original wideband-exciter DAPI set (that offers the highest brightness on the market - ideal for very low fluorophore concentrations).

Also new to the BrightLine product line is a new five-filter DAPI/FITC/Texas Red 'Pinkel' set (includes three single-band exciters), based on the popular and award-winning BrightLine multiband set.

With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, this new set is ideal for very high-speed imaging of blue, green, and red fluorophores using a low-noise monochrome camera.

'These filter sets have been developed specifically in response to the continued strong demand for high-performance and high-reliability fluorescence filters'.

'Semrock has realised this unprecedented level of performance with the implementation of our advanced, state-of-the-art ion beam sputtering (IBS) process'.

'With older technologies, the performance is often particularly compromised in the ultraviolet region of the spectrum, and hence we have received numerous requests for these filter sets as a direct follow-on to our existing products', explained Dr Turan Erdogan, CTO of Semrock.

Overall, Semrock's BrightLine fluorescence filters provide the highest peak transmission for maximum brightness while simultaneously providing superior contrast for high specificity, and are made exclusively with dense hard-coating technology without any adhesives in the optical path.

The unparalleled optical performance of patented BrightLine filters is made possible by combining the most modern ion-beam sputtering deposition systems with Semrock's proprietary optical-coating technology, for proven volume-manufacturing.

Fluorescence filters are widely used for applications including fluorescence microscopy, drug discovery, chemical analysis, DNA and RNA nucleotide sequencing, toxicology and blood analysis.

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