Semrock unveils fluorescence filter sets

Semrock has introduced a line of high-performance fluorescence filter sets that are optimised for microscopes and imaging systems that use multiple laser excitation.

A dual-band filter set for simultaneous 488 and 561nm laser excitation is ideal for the GFP and mCherry fluorescent protein combination, and a quad-band filter set for 405, 488, 561, and 635nm laser excitation ships in April.

Researchers with laser-based microscopes doing live-cell, fluorescent protein- based imaging - in which high sensitivity and high speeds are critical - will find the 488/561 dual-band set provides high-level brightness with laser blocking for high contrast.

This set is ideal for applications involving GFP and mRFP (especially mCherry) and similar fluorophore combinations It takes full advantage of high-end laser microscopes, and can be used to cost-effectively upgrade the performance of older systems.

The 488/561 set offers steep transitions from laser blocking to fluorescence transmission, high transmission (>93 per cent), and deep blocking (OD>6) at the laser wavelengths, to minimise the noise background resulting from residual excitation light.

The matched beamsplitter has superior reflection, rapid transitions and high-transmission, low-ripple fluorescence passbands, along with the flatness, low autofluorescence, and surface cosmetic properties desired for superior imaging performance.

The quad-band laser fluorescence set is designed for simultaneous 405/488/561/635 excitation wavelengths, and is optimal for DAPI/GFP/mCherry/Cy5 and similar fluorophore combinations.

These multi-laser sets are ideal for confocal microscopes such as the Olympus Fluoview FV300, FV1000 and the Nikon Eclipse CA Plus/si, AI; for total-internal-reflection-fluorescence (TIRF) systems such as the Nikon TIRF, Olympus FV1000-EVA and TIRF Illuminator, and for spinning-disk confocal microscopes, such as the Olympus DSU system.

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